Protective Effect of Genipin on Oxidative Stress Injury of Rpe Cells in High Glucose Environment
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Author(s)
Shi Yan, Chen Qingyou, Xu Wenshuang, Jia Di, Xu Jing
Corresponding Author
Shi Yan
Abstract
To study the antioxidant effect of Genipin (Gen), an extract from Gardenia jasminoides Ellis, on human Retinal Pigment Epithelium (RPE) cells injured by high glucose.RPE cell injury model was established invitro under high concentration of glucose (50 mmol / L), and the cells were cultured and detected in groups. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were measured by WST-1 method and enzyme labeling method, the content of catalase (CAT) was detected by microplate method, and the level of reactive oxygen species (ROS) was detected by fluorescence probe DCF.the content of MDA in the high glucose group (HG) was significantly lower than that in the Genipin plus high glucose group (HG + GEN) (P < 0.05), the activity of antioxidant enzymes SOD, the contents of CAT and GPX were significantly increased (P < 0.05), and the level of ROS detected by DCF fluorescence probe was decreased (P < 0.05).Genipin has protective effect on RPE cells damaged by high concentration glucose.
Keywords
Genipin, Diabetic retinopathy, Oxidative damage, High glucose
Acknowledgments
None
References
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