Protective Effect of Genipin on Oxidative Stress Injury of Rpe Cells in High Glucose Environment
Author(s)
Shi Yan, Chen Qingyou, Xu Wenshuang, Jia Di, Xu Jing
Corresponding Author
Shi Yan
Abstract
To study the antioxidant effect of Genipin (Gen), an extract from Gardenia jasminoides Ellis, on human Retinal Pigment Epithelium (RPE) cells injured by high glucose.RPE cell injury model was established invitro under high concentration of glucose (50 mmol / L), and the cells were cultured and detected in groups. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were measured by WST-1 method and enzyme labeling method, the content of catalase (CAT) was detected by microplate method, and the level of reactive oxygen species (ROS) was detected by fluorescence probe DCF.the content of MDA in the high glucose group (HG) was significantly lower than that in the Genipin plus high glucose group (HG + GEN) (P < 0.05), the activity of antioxidant enzymes SOD, the contents of CAT and GPX were significantly increased (P < 0.05), and the level of ROS detected by DCF fluorescence probe was decreased (P < 0.05).Genipin has protective effect on RPE cells damaged by high concentration glucose.
Keywords
Genipin, Diabetic retinopathy, Oxidative damage, High glucose
Acknowledgments
None
References
[1] Peng Jinjuan, Huang Jiannan, Lu Lina, et al. Research Progress of Remote Screening System for Diabetic Retinopathy. Chinese Journal of Ophthalmology, Vol.52, No.11, pp.868-871, 2016.
[2] Hou Lili, Chen Ting, Liu Liting, et al. Protective Effect of Growth Hormone Releasing Polypeptide on High Glucose Induced Retinal Pigment Epithelial Cells. International Journal of Ophthalmology, Vol.18, No.7, pp.1184-1187, 2018.
[3] Zheng Haiyan. Evaluation of Oxidative Stress Injury and Epithelial Mesenchymal Transition of Human Retinal Pigment Epithelial Cells under High Glucose. Journal of Hainan Medical College, Vol.23, No.4, pp.566-569, 2017.
[4] Fan Yan, Lu Hong, Hou Dingshan, et al. Proliferation Promoting and Anti apoptotic Effects of Sphingosine 1-phosphate on Human Retinal Pigment Epithelial Cells under Hypoxia [J]. Chinese Journal of Experimental Ophthalmology, Vol.33, No.1, pp.33-37, 2015.